Effects of a Novel Protease from Bacillus Subtilis K-5 in Low Protein Corn Distiller Dried Grains with Solubles (cDDGS) Based Diets on Performance and Nutrients Digestibility in Broiler Chickens

This experiment was conducted to evaluate the supplemental effects of a novel protease produced from Bacillus subtilis in low crude protein (CP) corn distiller dried grain with solubles (cDDGS) based diets on growth performance, carcass attributes, nutrients digestibility, blood chemistry, and intestinal histomorphometry broiler chickens. One hundred sixty, one-day-old chicks were randomly allotted one 4 dietary treatments. Each treatment had four replicates, 10 birds each replicate. Two basal formulated for both starter (1-21d) finisher (22-35d) phase; (PC) soybean meal diet as per standard recommendations Ross 308; (NC) 5% cDGGS reduction CP concomitant essential amino acids (EAAs) compared PC. The negative control further subdivided into 3 parts. part without enzyme supplementation, while other two parts supplemented (PROT1) commercial (PROT2), respectively. same procedure adopted diets. A digestibility assay (32-35d) carried out using acid insoluble ash (AIA), an external marker. At end 35d, ileal digesta collected experimental unit nutrient measurement. Tissue samples duodenum, jejunum, ileum villus height, width, crypt depth, width. Body weight gain (BWG) feed:gain improved (p<0.05) supplementation. No effect observed parameters. However, apparent coefficient nitrogen (ADCn), retention (Nret ), (AIAAD) (p<0.05). there no metabolizable energy (AME) corrected (AMEn), glucose, total cholesterol (p>0.05) integrity (p>0.05). It concluded that can improve resulting availability